Immobilization of biotinylated bacteriophages on biosensor surfaces
نویسندگان
چکیده
Immobilization of biotinylated bacteriophages on biosensor surfaces L. Gervais a, M. Gel a, B. Allain b, M. Tolba c, L. Brovko c, M. Zourob b, R. Mandeville b, M. Griffiths c, S. Evoy a,∗ a Department of Electrical and Computer Engineering and National Institute for Nanotechnonology, University of Alberta, Edmonton, T6G 2V4 Canada b Biophage Pharma Inc., 6100 Royalmount Montreal, Quebec, H4P 2R2 Canada c Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario, N1G 2W1 Canada
منابع مشابه
Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.
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